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d.

Cons/Weaknesses of the Manuscript:

Reviewer 1 (Achen):

The result tables and the figures do not provide a key to define the acronyms used for the

statistical analysis. The pages were not numbered making it difficult to refer to comments.

Reviewer 2 (Douey):

If this is considered the official method (maybe another manuscript will be written for all 24

food / environmental types?), needs more detail on enrichment for all 24 types.

Reviewer 3 (Hitchins):

1.

The tables tend to be too complex, repetitive and redundant.

2.

Performance parameters in Table 7 are not mentioned in the narrative.

Reviewer 4 (Brodsky):

None.

Reviewer 5 (Hammack):

1.

The official method is missing sample preparation procedures for a variety of matrices.

The pre-collaborative study, Section D (Sample Enrichment), has 25 different sample

preparation procedures. It may be possible to consolidate some of these, but they must

all appear (consolidated of not) in the official method since it is not possible to run the

method without them. This information should all appear in Section D of the official

method.

2. Section G, of the official method. Please include hyperlinks in the confirmation section,

since the official method is a standalone document. Also, this section is not a results

section, so phrases such as "was used" should not appear in the section.

3. There should be a reference section in the official method that refers to the method's

validation history. Please look in the OMA for guidance.

4. Page 1, line 21, abstract. Please specify the matrix type. For orange juice, was it

pasteurized or unpasteurized? Was it from concentrate? For frankfurters, what kind?

Beef, chicken, or turkey?

5. Page 2, line 27. Change "...statistical difference when..." to "statistical difference with

the reference method when..."

6. Page 2 line 34. What were the matrices (see comment 2 above)?

7. Page 3, lines 22-23. How long were the inocula stressed and what was the percent

injury. Please write in the report.

8. Page 3, lines 20-37. How long were the test portions aged after inoculation? This

includes sample shipment.

9. Page 8, line 4. Change “…but difficult…” to “…but was difficult…”