24
Biophysical Society 58
th
Annual Meeting, San Francisco, California
231-P
lat
4:15
pm
COMPRESSION, VOLUME AND PROLIFERATION ARREST.
Morgan Delarue
, Fabien Montel, Danijela Vignjevic, Jacques Prost,
Jean-François Joanny, Giovanni Cappello
232-P
lat
4:30
pm
THE EVOLUTION OF MECHANICAL PROPERTIES OF
DIFFERENTIATING STEM CELLS IS FATE- AND FUNCTION-
DEPENDENT. Andrew Ekpenyong,
Jochen Guck
233-P
lat
4:45
pm
VIEWING NUCLEAR DEFORMATION WITH SIDEWAYS
MICROSCOPY.
Kellie N. Beicker
, Timothy E. O’Brien,
Michael R. Falvo, Richard Superfine
234-P
lat
5:00
pm
IMAGING MECHANICAL FORCE TRANSMISSION AT SINGLE
INTEGRINS IN LIVING CELLS.
Masatoshi Morimatsu
,
Armen H. Mekhdjian, Alice Chang, Alexander R. Dunn
235-P
lat
5:15
pm
THE ROLE OF ARP2/3 IN DRG GROWTH CONES MOTILITY.
Wasim A. Sayyad
, Paolo Fabris, Jelena Ban, Erika Ercolini, Vincent Torre
236-P
lat
5:30
pm
PROBING CELL MEMBRANE MECHANICS BY MAGNETIC
PARTICLE ACTUATION AND 3D ROTATIONAL PARTICLE
TRACKING. Matthias Irmscher, Arthur M. de Jong,
Holger Kress
,
Menno W.J Prins
237-P
lat
5:45
pm
TIGHT COUPLING BETWEEN NUCLEUS AND CELL MIGRATION
THROUGH THE PERINUCLEAR ACTIN CAP.
Dong-Hwee Kim
,
Denis Wirtz
5:00
pm
–6:30
pm
, R
oom
123
Exhibitor Presentation
Asylum Research, an Oxford Instruments
Company
New blueDrive™ Photothermal Excitation for Superior AFM Tapping
Mode Imaging
Asylum Research, an Oxford Instruments company, will introduce its
new blueDrive Photothermal Excitation capabilities exclusively avail-
able on Cypher™, the highest resolution fast scanning AFM. blueDrive
significantly enhances the performance of tapping mode imaging with
more simple, stable and quantitative operation, and providing extremely
clean tunes in both air and water. Typically, a piezoacoustic excitation
has been used to drive the cantilever oscillation. Though piezo drive is
favored for design simplicity, the response of the cantilever is often far
from ideal, causing users to spend countless time selecting a clean canti-
lever tune. Asylum’s blueDrive excitation mechanism produces an almost
perfect response by directly exciting the cantilever photothermally with
a blue laser. blueDrive is ideal for high resolution imaging of biological
samples in fluid including proteins, lipids and nucleic acids, as well as
force measurements and nanomechanics. In this presentation, we will
explain how blueDrive works, how it achieves simple cantilever tunes,
and show real world results for biophysics applications.
Presenter:
Nick Geisse, Applications Scientist, Asylum Research, an Oxford
Instruments company
5:00
pm
–8:00
pm
, R
oom
307
Korean Biophysicists Meeting
6:00
pm
–7:00
pm
, R
oom
121
Biophysics Austria Mixer
6:00
pm
–7:30
pm
, R
oom
302
Biophysical Society of Canada–
Travel Awards and Mixer
6:00
pm
–9:00
pm
, H
all
D
Student Research Achievement Award
(SRAA) Poster Competition
This session features students who are presenting posters at the Meeting
and have pre-registered for the competition. During the competition,
students give a five-to-seven minute verbal presentation of their poster to
one or more judges. Winners will be recognized on Monday evening prior
to the National Lecture.
7:00
pm
–8:30
pm
, R
oom
123
Exhibitor Presentation
FEI Company
Cryo-TEM: A New Era for 3D Structural Analysis of Protein Complexes
A new frontier exists in unraveling interactive biological and biochemical
processes and pathways at the macromolecular level. Of critical impor-
tance is the three-dimensional visualization of macromolecular structures
and molecular machines in their native functional state. Three techniques
play a major role in orchestrating this.
Nuclear magnetic resonance (NMR) has the capability to study specific
protein domains or fragments and their functional role in protein fold-
ing and dynamics and in ligand binding whereas X-Ray crystallography
(XRD) allows visualizing high-resolution but more static 3D structures of
apo and liganded proteins, mainly in a monomeric or dimeric state after
crystallization. To unravel more physiologically relevant situations how-
ever, it is essential to visualize multimeric complexes in their tertiary and
quaternary state and their interaction with other complexes. By perform-
ing typical cryo-TEM applications like single particle analysis or tomog-
raphy, this can be achieved. In this so-called translational methodology,
cryo-TEM thus provides complementary information to NMR and XRD
that can be crucial for drug discovery, e.g. in terms of a better understand-
ing of the mechanism of action inferred from the EM structure of the
physiologically relevant complex. This will eventually contribute to answer
real biologically as well as medically relevant questions. Latest develop-
ments in the cryo-TEM workflow have brought the three major structural
biology technologies closer together. Now, finally, a continuum has been
reached on all important aspects with regards to resolution and macromo-
lecular scales which allows for the full deployment of the combination of
these technologies.
Here, we will illustrate the historical context of these technologies with
respect to one another and show how latest developments have reached
the critical requirements to fully unleash the power of structural biology
in not just answering fundamental questions, but actually contribute to
curing diseases and improving health. Also, we will discuss the future of
structural biology based on the latest developments of the FEI workflow
and its components.
Presenters:
Marc Storms, Marketing Manager, Life Sciences, FEI Company
Jeff Lengyel, Product Marketing Manager, FEI Company
Eric Hnath, Product Marketing Manager, Structural Biology, FEI Company
Thomas Wohlfarth, Director, Structural Biology Businesses, FEI Company
