19
Biophysical Society 58
th
Annual Meeting, San Francisco, California
S
A
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F
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10:45
am
–12:45
pm
, R
oom
305
Platform
Exocytosis and Endocytosis
Co-Chairs
Jenny Hinshaw, NIDDK, NIH
Diego Krapf, Colorado State University
168-P
lat
10:45
am
withdrawn
169-P
lat
11:00
am
MOLECULAR DYNAMICS SIMULATIONS OF SNARE COMPLEX
UNZIPPING.
Satyan Sharma
, Manfred Lindau
170-P
lat
11:15
am
CHOLESTEROL PROMOTES OPENING OF THE SNARE-
MEDIATED FUSION PORE.
Benjamin S. Stratton
, Zhenyong Wu,
Jason M. Warner, George Wei, Emma C. Wagnon, Erdem Karatekin,
Ben O’Shaughnessy
171-P
lat
11:30
am
PROTEIN MOBILITY IN SECRETORY GRANULES AND FUSION
PORE EXPANSION: FACTORS AFFECTING PROTEIN
SECRETION.
Annita Ngatchou-Weiss
, Mary A. Bittner,
Arun Anantharam, Daniel Axelrod, Ronald W. Holz
172-P
lat
11:45
am
NANOSTRUCTURE-INDUCED MEMBRANE CURVATURE
RECRUITS ENDOCYTOSIS MACHINARY IN LIVING CELLS.
Wenting Zhao
, Lindsey Hanson, Ziliang Lin, Yi Cui, Bianxiao Cui
173-P
lat
12:00
pm
A DYNAMIN MUTANT DEFINES A SUPER-CONSTRICTED
PRE-FISSION STEP.
Anna Sunborger
, Jurgen A. Heyman,
Shunming Fang, Joshua S. Chappie, Jenny E. Hinshaw
174-P
lat
12:15
pm
CLATHRIN AGGREGATION BY ROTATIONAL BROWNIAN
DYNAMICS.
Ioana M. Ilie
, Wouter K. den Otter, Wim J. Briels
175-P
lat
12:30
pm
QUANTIFYING THE DYNAMIC INTERACTIONS BETWEEN
A CLATHRIN-COATED PIT AND CARGO MOLECULES.
Aubrey V. Weigel, Michael M. Tamkun,
Diego Krapf
10:45
am
–12:45
pm
, R
oom
306
Platform
Cardiac Muscle I
Co-Chairs
Steven Schwartz, University of Arizona
Gerrie Farman, Boston University
176-P
lat
10:45
am
A REVISED ATOMISTIC MODEL OF THE CARDIAC THIN
FILAMENT AND APPLICATION TO A SPECIFIC DISEASE
CAUSING MUTATION.
Michael R. Williams
, Jil Tardiff,
Steven Schwartz
177-P
lat
11:00
am
MOLECULAR MECHANISM FOR THE REGULATION OF
CARDIAC MUSCLE CONTRACTION BY TROPONIN.
Ivanka Sevrieva, Andrea Knowles,
Yin-Biao Sun
178-P
lat
11:15
am
CALCIUM-SENSITIVE DYNAMIC EFFECTS OF TROPONIN’S
TNI INHIBITORY REGION. Julie Mouannes Kozaili,
Devanand Kowlessur,
Larry S. Tobacman
179-P
lat
11:30
am
DYNAMIC EFFECTS OF TROPOMYOSIN D230N MUTATION
AND FETAL TROPONIN T ON THE TROPOMYOSIN OVERLAP
REGION.
Mark T. McConnell
, Jayant J. Jayasundar, Lauren Grinspan,
Ofer Z. Fass, Benjamin Schwartz, Jil C. Tardiff
180-P
lat
11:45
am
IN SITU STRUCTURAL CHANGES IN THICK AND THIN
FILAMENTS OF CARDIAC MUSCLE INDUCED BY FRAGMENTS
OF MYOSIN BINDING PROTEIN C (MYBP-C).
Thomas Kampourakis
, Yin-Biao Sun, Mathias Gautel, Malcolm Irving
181-P
lat
12:00
pm
IMPACT OF FAMILIAL HYPERTROPHIC CARDIOMYOPATHY-
LINKED MUTATIONS IN THE N-TERMINUS OF THE MYO-
SIN REGULATORY LIGHT CHAIN ON THE CALCIUM BASED
MOTILITY.
Gerrie P. Farman
, Priya Mutha, Katarzyna Kazmierczak,
Danuta Szczesna-Cordary, Jeffery R. Moore
182-P
lat
12:15
pm
PHOSPHORYLATION MODULATES THE DYNAMICS OF THE
N-TERMINAL TAIL IN CARDIAC RLC.
Arianna Fornili
,
Elena Rostkova, Franca Fraternali, Mark Pfuhl
183-P
lat
12:30
pm
FAMILIAL HYPERTROPHIC CARDIOMYOPATHY-LINKED
MUTATION (K104E) IN THE MYOSIN REGULATORY LIGHT
CHAIN AFFECTS SARCOMERIC STRUCTURE AND FUNCTION
IN TG-MICE.
Wenrui Huang
, Jingsheng Liang, Katarzyna Kazmierczak,
Priya Muthu, Chen-Ching Yuan, Ana I. Rojas, Divya Duggal,
Julian Borejdo, Thomas C. Irving, Danuta Szczesna-Cordary
11:00
am
–12:00
pm
, R
oom
122
International Relations Committee Meeting
11:00
am
–12:30
pm
, R
oom
123
Exhibitor Presentation
Molecular Devices, LLC
Investigating Use-Dependent Inhibition of Ion Channels on Auto-
mated Electrophysiology Systems including the IonWorks Barracuda
®
Instrument and the IonFlux
TM
Benchtop Reader
Use- dependent inhibition of ion channels by potential drug candidates is
an important aspect to investigate for many drug classes. Use-dependent
drugs specifically target ion channels in cells that are more electrically
active. For example, a drug targeting pain that is more potent to Na
+
chan-
nels in neurons actively firing action potentials is a better drug candidate.
Data will be presented to demonstrate the ability of automated electro-
physiology systems to study the use-dependence block of Na
+
channel
targets. Tetracaine, lidocaine, and TTX exhibit very different behavior in
terms of their use- dependent blockage. We will demonstrate the ability
of the instrumentation to deliver complex voltage protocols and gener-
ate long assay windows which are required for these studies. Pulse trains
delivered at 10Hz are used to measure the blockade of current. Data from
a separate study will also be presented that demonstrate blockage and
enhancement of NaV1.5 currents by various peptide toxins. Both sets of
experiments demonstrate stable assay windows with uniform currents for
30 minutes and longer during the delivery of periodic pulse trains.
Presenter:
James Costantin, Product Marketing Manager, Automated Electrophysiology,
Molecular Devices, LLC
