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procedure. There were 3 observed positives for the reference method. For the high inoculation
1
level, there were 3 presumptive positives and 3 confirmed positives following the FDA/BAM
2
Chapter 10 reference method confirmation procedure. There were 4 confirmed positives
3
following the reference method. Detailed results of the POD analyses are presented in Tables 8
4
and 12. This matrix analysis was conducted using Demi Fraser with FAC brand X.
5
6
7
Compared to ISO 11290-1/A1
8
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 6 presumptive positives
9
and 6 confirmed positives following the ISO 11290-1/A1 reference method confirmation
10
procedure. There were 9 observed positives for the reference method. For the high inoculation
11
level, there were 3 presumptive positives and 3 confirmed positives following the ISO 11290-
12
1/A1 reference method confirmation procedure. There were 4 confirmed positives following the
13
reference method. Detailed results of the POD analyses are presented in Tables 8 and 12
14
This matrix analysis was conducted using Demi Fraser with FAC brand X.
15
16
Cold smoked salmon
17
18
Compared to FDA-BAM
19
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 10 presumptive positives
20
and 10 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation
21
procedure. There were 7 observed positives for the reference method. For the high inoculation
22
level, there were 5 presumptive positives and 5 confirmed positives following the FDA/BAM
23
Chapter 10 reference method confirmation procedure. There were 4 confirmed positives
24
following the reference method. Detailed results of the POD analyses are presented in Tables 8
25
and 12. This matrix analysis was conducted using Demi Fraser with FAC brand X.
26
27
28
29
Compared to ISO 11290-1/A1
30
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 10 presumptive positives
31
and 10 confirmed positives following the ISO 11290-1/A1 reference method confirmation
32
procedure. There were 8 observed positives for the reference method. For the high inoculation
33
level, there were 5 presumptive positives and 5 confirmed positives following the ISO 11290-
34
1/A1 reference method confirmation procedure. There were 4 confirmed positives following the
35
reference method. Detailed results of the POD analyses are presented in Tables 8 and 12.
36
This matrix analysis was conducted using Demi Fraser with FAC brand X.
37
38
39
40
Independent Study using Demi Fraser with FAC brand Y.
41
42
Deli turkey (125 g)
43
44
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 8 presumptive positives
45
and 8 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
46
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 D/ PTM Validation Report 111501
OMA ERP - June 2016
ERP Use Only