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reference method confirmation procedure. There were 5 confirmed positives following the

1

reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

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3

4% Milk fat cottage cheese (25 g)

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5

For the low inoculation level of the MDA 2 -

Listeria

assay, there were 12 presumptive positives

6

and 12 confirmed positives following the AOAC 993.12 reference method confirmation

7

procedure. There were 9 observed positives for the reference method. For the high inoculation

8

level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12

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reference method confirmation procedure. There were 5 confirmed positives following the

10

reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

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12

13

Beef hot dog (25 g)

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15

For the low inoculation level of the MDA 2 -

Listeria

assay, there were 4 presumptive positives

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and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation

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procedure, resulting in 1 false negative. There were 6 observed positives for the reference

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method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed

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positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There

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were 5 confirmed positives following the reference method. Detailed results of the POD analyses

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are presented in Tables 9 and 13.

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23

24

Stainless steel (sponge enriched in 225 mL)

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26

Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level

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of the MDA 2 -

Listeria

assay, there were 5 presumptive positives and 5 confirmed positives

28

following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 8

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observed positives for the reference method. For the high inoculation level, there were 5

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presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference

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method confirmation procedure. There were 5 confirmed positives following the reference

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method. Detailed results of the POD analyses are presented in Tables 9 and 13.

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Sealed concrete (sponge enriched in 100 mL)

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Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level

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of the MDA 2 -

Listeria

assay, there were 15 presumptive positives and 15 confirmed positives

39

following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 11

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observed positives for the reference method. For the high inoculation level, there were 5

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presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference

42

method confirmation procedure. There were 5 confirmed positives following the reference

43

method. Detailed results of the POD analyses are presented in Tables 9 and 13.

44

45

Plastic (Enviroswab enriched in 10 mL)

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AOAC Research Institute

Expert Review Panel Use Only

OMAMAN-29 D/ PTM Validation Report 111501

OMA ERP - June 2016

ERP Use Only