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reference method confirmation procedure. There were 5 confirmed positives following the
1
reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.
2
3
4% Milk fat cottage cheese (25 g)
4
5
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 12 presumptive positives
6
and 12 confirmed positives following the AOAC 993.12 reference method confirmation
7
procedure. There were 9 observed positives for the reference method. For the high inoculation
8
level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12
9
reference method confirmation procedure. There were 5 confirmed positives following the
10
reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.
11
12
13
Beef hot dog (25 g)
14
15
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 4 presumptive positives
16
and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation
17
procedure, resulting in 1 false negative. There were 6 observed positives for the reference
18
method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed
19
positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There
20
were 5 confirmed positives following the reference method. Detailed results of the POD analyses
21
are presented in Tables 9 and 13.
22
23
24
Stainless steel (sponge enriched in 225 mL)
25
26
Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level
27
of the MDA 2 -
Listeria
assay, there were 5 presumptive positives and 5 confirmed positives
28
following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 8
29
observed positives for the reference method. For the high inoculation level, there were 5
30
presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference
31
method confirmation procedure. There were 5 confirmed positives following the reference
32
method. Detailed results of the POD analyses are presented in Tables 9 and 13.
33
34
35
Sealed concrete (sponge enriched in 100 mL)
36
37
Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level
38
of the MDA 2 -
Listeria
assay, there were 15 presumptive positives and 15 confirmed positives
39
following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 11
40
observed positives for the reference method. For the high inoculation level, there were 5
41
presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference
42
method confirmation procedure. There were 5 confirmed positives following the reference
43
method. Detailed results of the POD analyses are presented in Tables 9 and 13.
44
45
Plastic (Enviroswab enriched in 10 mL)
46
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29 D/ PTM Validation Report 111501
OMA ERP - June 2016
ERP Use Only