173 / 596
procedure. There were 7 observed positives for the reference method. For the high inoculation
1
level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS
2
MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives
3
following the reference method. Detailed results of the POD analyses are presented in Tables 9
4
and 13.
5
6
Naturally Contaminated raw chicken leg pieces (25 g)
7
8
Lot 1
9
For lot 1 of the raw chicken leg pieces of the MDA 2 -
Listeria
assay, there were 18 presumptive
10
positives and 18 confirmed positives following the USDA/FSIS MLG 8.09 reference method
11
confirmation procedure. There were 16 observed positives for the reference method. Detailed
12
results of the POD analyses are presented in Tables 9 and 13.
13
14
Lot 2
15
For lot 2 of the raw chicken leg pieces of the MDA
Listeria -
2 assay, there were 12 presumptive
16
positives and 12 confirmed positives following the USDA/FSIS MLG 8.09 reference method
17
confirmation procedure. There were 14 observed positives for the reference method. Detailed
18
results of the POD analyses are presented in Tables 9 and 13.
19
20
Whole melon
21
22
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 12 presumptive positives
23
and 10 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation
24
procedure, resulting in 2 false positives. There were 8 observed positives for the reference
25
method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed
26
positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There
27
were 5 confirmed positives following the reference method. Detailed results of the POD analyses
28
are presented in Tables 9 and 13.
29
30
Vanilla ice cream (25 g)
31
32
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 7 presumptive positives
33
and 7 confirmed positives following the AOAC 993.12 reference method confirmation
34
procedure. There were 7 observed positives for the reference method. For the high inoculation
35
level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12
36
reference method confirmation procedure. There were 5 confirmed positives following the
37
reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.
38
39
Queso fresco (25 g)
40
41
For the low inoculation level of the MDA 2 -
Listeria
assay, there were 11 presumptive positives
42
and 11 confirmed positives following the AOAC 993.12 reference method confirmation
43
procedure. There were 9 observed positives for the reference method. For the high inoculation
44
level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12
45
AOAC Research Institute
Expert Review Pan l Use Only
OMAMAN-29 D/ PTM Validation Report 111501
OMA ERP - June 2016
ERP Use Only