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Mass Spectrometry (MS)
Di
MS i h h
hi
i
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rect
w t out c romatograp c separat on
(Ambient MS or Flow‐Injection MS)
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GC‐MS – limited use for identification
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LC‐MS – prevalent technique for PDE5 inhibitors
Benefits of MS:
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Sensitivity
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Selectivity – tandem MS (MS/MS) and accurate mass‐
high resolution (AM/HR) MS and MS/MS
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Structure‐based identification/confirmation
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Can be combined with UV or fluorescence detection
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Suitable for both targeted and non‐targeted analysis