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Spectrofluorometry
•
Measurement based on excitation and emission
maxima of known PDE5 inhibitors
P i l f
i
d (
i )
i i
•
otent a or screen ng an sem ‐ quant tat on
•
Sensitivity and linearity can be improved by
addition of surfactants (demonstrated for sildenafil)
Examples:
Wang et al Anal Methods 2 (2010) 519 524
‐
.,
.
–
‐
Anumolu et al., Asian J. Pharm. Clin. Res. 6 (2013)326‐329