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17
Raw Ground Beef –
mericon
E. coli
O157
Screen Plus with Automated Preparation
1
For the low inoculum level, 69 out of 144 test portions (POD
CP
of 0.48) were reported as
2
presumptive positive by the
mericon
E. coliO157Screen Plus with 65 out of 144 test portions
3
(POD
CC
& POD
R
of 0.45) confirming positive. For samples that produced presumptive positive
4
results on the
mericon
E. coli O157Screen Plus, 65 out of 144 samples confirmed positive
5
(POD
C
of 0.45). A dLPOD
C
value of 0.00 with 95% confidence interval of (-0.12, 0.12) was
6
obtained between the candidate and reference method, indicating no statistically significant
7
difference between the two methods. A dLPOD
CP
value of 0.03 with 95% confidence intervals of
8
(-0.09, 0.15) was obtained between presumptive and confirmed results indicating no statistically
9
significant difference between the presumptive and confirmed results.
10
11
For the high inoculum level, 144 out of 144 test portions (POD
CP
of 1.00) were reported as
12
presumptive positive by the
mericon
E. coliO157Screen Plus with 144 out of 144 test portions
13
(POD
CC
& POD
R
of 1.00) confirming positive. For samples that produced presumptive positive
14
results on the
mericon
E. coli O157Screen Plus, 144 out of 144 samples confirmed positive
15
(POD
C
of 1.00). A dLPOD
C
value of 0.00 with 95% confidence interval of (-0.03, 0.03) was
16
obtained between the candidate and reference method, indicating no statistically significant
17
difference between the two methods. A dLPOD
CP
value of 0.00 with 95% confidence intervals of
18
(-0.03, 0.03) was obtained between presumptive and confirmed results indicating no statistically
19
significant difference between the presumptive and confirmed results.
20
21
For the un-inoculated controls, 2 out of 144 samples (POD
CP
of 0.01) produced a presumptive
22
positive result by the
mericon
E. coliO157Screen Plus with 0 out of 144 test portions (POD
CC
&
23
POD
R
of 0.00) confirming positive. For samples that produced presumptive positive results on
24
the
mericon
E. coliO157Screen Plus, 0 out of 144 samples confirmed positive (POD
C
of 0.00).
25
A dLPOD
C
value of 0.00 with 95% confidence interval of (-0.03, 0.03) was obtained between the
26
candidate and reference method, indicating no statistically significant difference between the two
27
methods. A dLPOD
CP
value of 0.01 with 95% confidence intervals of (-0.01, 0.05) was obtained
28
between presumptive and confirmed results indicating no statistically significant difference
29
between the presumptive and confirmed results.
30
31
Detailed results of the POD statistical analysis are presented in Table 2016.2Cand Figures 3A-
32
3B.
33
34
Raw Ground Beef –
mericon
E. coliSTEC O-Type with Automated Preparation
35
For the low inoculum level, 63 out of 144 test portions (POD
CP
of 0.44) were reported as
36
presumptive positive by the
mericon
E. coliSTEC O-Type with 65 out of 144 test portions
37
(POD
CC
& POD
R
of 0.45) confirming positive. For samples that produced presumptive positive
38
results on the
mericon
E. coliSTEC O-Type, 63 out of 144 samples confirmed positive (POD
C
of
39
0.44). A dLPOD
C
value of -0.01 with 95% confidence interval of (-0.13, 0.10) was obtained
40
between the candidate and reference method, indicating no statistically significant difference
41
between the two methods. A dLPOD
CP
value of -0.01 with 95% confidence intervals of (-0.13,
42
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only