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17) Close the “Eluate and Reagents” and “Assays” drawers.
1
2
18) Upon closing each drawer, select “Yes” to start the inventory scan for each drawer.
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4
19) Select “Queue”. Monitoring of the cooling starts.
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6
20) Select “Run” to start the run.
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8
21) After the run is finished, select “Remove” in the assay setup “Overview” screen.
9
Open the “Assays” drawer and unload the Rotor-Disc 72.
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11
22) Place the Rotor-Disc sealing film over the Rotor-Disc 72; place it into the Rotor-
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Disc heat sealer. Sealing is complete in about 2 minutes. Remove the excess film
13
from the Rotor-Disc 72 and transfer to the Rotor-Disc 72 Rotor. In the “Sample
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Rack Layout” screen of the assay setup user interface, the elution rack in slot 2 is
15
pictured.
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F.
Analysis
17
(a)
Prepare Equipment
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19
1)
Turn on the Rotor-Gene Q instrument and attached computer. Load the Rotor-Gene
20
Q Series Software. Start an empty run to warm up the Rotor-Gene Q.
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22
i.
Run a Disc
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ii.
Load Samples
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25
2)
Manual
mericon
DNA Extraction Method
26
27
i.
Load the QIAGEN strip tubes into the 72-Well Rotor strip tube ring. Snap
28
the locking ring in place over the strip tubes, and place into the Rotor-
29
Gene Q. When running strip tubes, balance the ring using the empty strip
30
tubes.
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3)
Automated QIAsymphonyDNA Extraction Method
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34
i.
Load the QIAGEN 72 Rotor-Disk into the 72-Rotor Disk ring. Snap the
35
locking ring in place over the Rotor Disk, and place into the Rotor-Gene
36
Q.
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38
4)
Start Instrument Run
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40
i.
Software Version 2.3.1 (Build 49)
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42
Follow the Rotor-Gene software instructions. Verify that the correct
43
setting is selected for each preparation method: the Rotor-Disc 72 rotor is
44
selected for the QIAsymphony (automated preparation method), and the
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OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only