222 / 453
10
1
10) Prepare the appropriate number of strip tubes by transferring 10 µL of reconstituted
2
assay to reaction tubes. Pipette 10 µL of extracted sample DNA into each sample
3
strip tube and mix by pipetting up and down 2-3 times.
4
5
11) Prepare the positive control tube by pipetting 10 µL of the Positive Control DNA
6
into a strip tube containing 10 µL of reconstituted assay. Prepare the negative
7
control by pipetting 10 µL of RNase-free water into a strip tube with 10 µL of
8
reconstituted assay.
9
10
12) Seal the strip tubes with the strip tube caps.
11
12
13) Load sample tubes into the 72-well rotor and load into the Rotor-Gene-Q and
13
initiate analysis immediately.
14
15
(b)
Automated QIAsymphony
®
DNA Extraction Method
16
17
1)
Ensure the “Waste” drawer is prepared properly, and perform an inventory scan of
18
the “Waste” drawer, including the tip chute and liquid waste. Replace the tip
19
disposal bag, if necessary.
20
21
2)
Load an elution microtube rack (EMTR) into the “Eluate” drawer and perform an
22
inventory scan of the “Eluate” drawer.
23
24
3)
Load the QIAsymphony
mericon
Bacteria Kit and consumables into the “Reagents
25
and Consumables” drawer. Select the “R+C” button in the touchscreen to open the
26
screen that shows the consumables status (“Consumables/8-RodCovers/Tubes/
27
Filter-Tips/Reagent Cartridges”). Select the “Scan Bottle” button to scan the bar
28
code of the TopElute bottle with the handheld bar code scanner. Select the “OK”
29
button.
30
4)
Perform an inventory scan of the “Reagents and Consumables” drawer.
31
32
5)
Transfer 500 µL of the enriched sample into a 2 mL screw cap tube. Place the
33
samples into the appropriate tube carrier and load them into the “Sample” drawer.
34
35
6)
Using the touch screen, enter the required information for each batch of samples to
36
be processed.
37
38
7)
Choose elution volumes according to Table B.
39
40
41
42
43
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only