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spilled, clean with a suitable laboratory detergent and water. If the spilled liquid contains
1
potentially infectious agents, clean the affected area first with laboratory detergent and
2
water, and then with 1% (v/v) sodium hypochlorite solution. Reagents in this kit are
3
highly flammable and are harmful by inhalation, skin contact and swallowing.
4
Real-Time PCR System
5
Improper use of the Rotor-Gene Q may cause personal injuries or damage to the
6
instrument. The Rotor-Gene Q must only be operated by qualified personnel who
7
have been appropriately trained. Servicing of the Rotor-Gene Q must only be
8
performed by QIAGEN Field Service Specialists.
9
Enrichment
10
Shiga-toxin producing
Escherichia coli
is a Biosafety Level 2 organism. Biological
11
samples such as enrichments have the potential to transmit infectious diseases. Follow
12
all applicable local, state/provincial, and/or national regulations on disposal of
13
biological wastes. Wear appropriate protective equipment which includes but is not
14
limited to: protective eyewear, face shield, clothing/lab coat, and gloves. All work
15
should be conducted in properly equipped facilities utilizing the appropriate safety
16
equipment (for example, physical containment devices). Individuals should be trained
17
in accordance with applicable regulatory and company/institution requirements before
18
working with potentially infectious materials. All enrichment broths should be
19
sterilized following any culture based confirmatory steps.
20
The user should read, understand and follow all safety information in the instructions
21
for the
mericon
E. coliO157 Screen Plus and
mericon
E. coliSTEC O-TypePathogen
22
Detection Assays. Retain the safety instructions for future reference.
23
To reduce the risks associated with exposure to chemicals and biohazards:
24
Performpathogen testing in a properly equipped laboratory under the control of
25
trained personnel.Always follow standard laboratory safety practices, including
26
wearing appropriate protective apparel and eye protection while handling reagents
27
and contaminated samples.Avoid contact with the contents of the enrichment media
28
and reagent tubes after amplification. Dispose of enriched samples according to
29
current industry standards.
30
When testing is complete, follow current industry standards for the disposal of
31
contaminated waste. Consult the Safety Data Sheet for additional information and
32
local regulations for disposal.
33
To reduce the risks associated with environmental contamination:Follow current
34
industry standards for disposal of contaminated waste.
35
D.
Sample Enrichment
36
37
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only