219 / 453
7
(p)
Elution Microtubes CL with cap strips (cat. 19588)
1
(q)
Cooling Adapter, Reagent Holder 1, (cat. 9018090)
2
(r)
Adapter 2 x Rotor-Disc, Qsym (cat. 9242204)
3
(s)
Rotor-Disc 72 Loading Block (cat. 9018910)
4
(t)
Rotor-Disc Heat Sealing Film (cat. 981604 (600) / 981601 (60))
5
(u)
Rotor-Disc Heat Sealer(cat. 9018898 (110V);
QIAGEN
cat. 9019725 (230V))
6
(v)
Filter-Tips, 200 µL (cat. 990332)
7
(w)
Filter-Tips, 50 µL (cat. 997120)
8
(x)
Disposable pipette – capable of 10-1000 µL
9
(y)
Sterile filter tip pipette tips- capable of 10-1000 µL
10
(z)
Top loading balance
–
Capable of weighing and measuring 25-2000 grams
11
(aa)
Filter Stomacher® bags
–
Seward or equivalent
.
12
(bb)
Stomacher®3500 – Seward or equivalent.
13
(cc)
Thermometer
–
calibrated range to include 100 ± 1
o
C
14
(dd)
Dry block heater unit
–
capable of maintaining 100 ± 1
o
C
15
(ee)
Microcentrifuge
–
Capable of holding 1.5-2 mL microcentrifuge tubes
16
(ff)
Vortex – For sample mixing
17
(gg)
Incubators
.
– Capable of maintaining 42 ± 1°C.
18
(hh)
Freezer –capable of maintaining -10 to -20
o
C, for storing the purified DNA for
19
long term storage and purified genomic DNA
20
(ii)
Refrigerator
–
capable of maintaining 2-8
o
C, for storing the purified viral DNA
21
for up to 24 hours prior to analysis
22
23
C. General Instructions
24
25
(a)
Store the
mericon
E. coliO157 Screen Plus and
mericon
E. coliSTEC O-TypePathogen
26
Detection Assaysat 2-8°C. Do not freeze. Keep kit away from light during storage.
27
After opening the kit, check that the foil pouch is undamaged. If the pouch is
28
damaged, do not use. After opening, unused reagent tubes should always be stored in
29
the re-sealable pouch with the desiccant inside to maintain stability of the lyophilized
30
reagents. Store the
mericon
E. coliPCR kit at -30 °C to -15 °C. Do not use either of
31
the
mericon
kits past the expiration date.
32
(b)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
33
Safety Precautions
34
mericon
E.coli O157 Screen Plusand
mericon
E.coli STEC O-Type
Pathogen
35
Detection Assays
and
mericon
DNA Bacteria Kit
36
All chemicals should be considered potentially hazardous. When working with
37
chemicals, always wear a suitable lab coat and disposable gloves. Product usage
38
should follow good laboratory practices.
39
QIAsymphony
mericon
Bacteria Kit
40
The buffers in the reagent cartridge contain guanidine salts, which can form highly
41
reactive compounds when combined with bleach. If liquid containing these buffers is
42
OMAMAN-36 A : Collaborative Study Manuscript
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only