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E. coli Detection Workflows 8/2015
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4.
Select the correct rotor and confirm the locking ring is attached by
checking the check box. Click “Next” to continue.
5.
Ensure that the reaction volume is set to 20 µl.
6.
Click “Next” to continue.
7.
Click “Edit Profile” and program the Rotor-Gene Q according to Table 9
Data acquisition should be performed during the annealing step at 60°C.
OMAMAN-36 C: Method User Guide/Package Insert
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only