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Table 22: Robustness Results for the
mericon
E. coli STEC O-Type Pathogen Detection Assay
Treatment
Combination
1
2
3
4
5
6
7
8
9
Incubation Time
8 Hours
8 Hours
8 Hours
8 Hours
10 Hours
10 Hours
10 Hours
10 Hours
9 Hours
Lysis Time
8 Minutes
8 Minutes
12 Minutes
12 Minutes
8 Minutes
8 Minutes
12 Minutes
12 Minutes
10 Minutes
1
MM:DNA Ratio (µL)
12:8
8:12
12:8
8:12
12:8
8:12
12:8
8:12
10:10
Sample #
Inoculated
1
-
-
-
-
+
+
+
+
+
2
-
-
-
-
+
+
+
+
+
3
-
-
-
-
-
-
-
-
-
4
-
-
-
-
+
+
+
+
+
5
-
-
-
-
-
-
-
-
-
6
-
-
-
-
+
+
+
+
-
7
-
-
-
-
-
-
-
-
-
8
-
-
-
-
+
+
+
+
-
9
-
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
Total Positives
0/10
0/10
0/10
0/10
5/10
5/10
5/10
5/10
3/10
Sample #
Uninoculated
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
Total Positives
0/5
0/5
0/5
0/5
0/5
0/5
0/5
0/5
0/5
1
MM= Master Mix
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only