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Figure 3: Summary Rotor-Gene Q instrument variation
Figure 3
:
Ct values for
Salmonella abony, Salmonella enteritidis, C. freundii
and the internal
control were not significantly different, indicating that there was no effect of the Rotor-Gene Q
instruments on the results obtained
Figure 4: Primer/probe and Master Mix lot consistency study with Automated
Bacteria DNA Preparation
Figure 4
:
Ct values for all primer/probe (PP) and master mix (MM) lot combinations for
Salmonella abony, Salmonella enteritidis, and C. freundii
were not significantly different,
indicating that there were no effects of individual lots of primer/probes and master mix on the
detection results
0
10
20
30
40
Salmonella abony
Salmonella enteritidis
Citrobacter freundii
Internal control
Ct
values
Cycler 1
Cycler 2
0
5
10
15
20
25
30
35
40
Salmonella abony Salmonella enteritidis Citrobacter freundii
Internal control
Ct value
PP1/MM1
PP2/MM1
PP3/MM1
PP1/MM2
PP2/MM2
PP3/MM2
PP1/MM3
PP2/MM3
PP3/MM3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research I stitute
Expert Review Panel Use Only