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APPENDIX 2
Salmonella spp
workflow
Stability and lot-to-lot variation
Figure 1. Primer/probe and Master Mix lot consistency study with Manual
Bacteria DNA Preparation
Figure1
:
Ct values for all primer/probe (PP) and master mix (MM) lot combinations for
Salmonella abony, Salmonella enteritidis, C. freundii
and the internal control were not
significantly different, indicating that there were no effects of individual lots of primer/probes and
master mix on the detection results
Figure 2. Summary: Lot consistency study with Manual Bacteria DNA Preparation
Figure 2
:
Ct values for
Salmonella abony, Salmonella enteritidis, C. freundii
and the internal
control were not significantly different, indicating that there were no effects of the lot of the
mericon
Fast Lysis Buffer
0
5
10
15
20
25
30
35
Salmonella abony Salmonella enteritidis Citrobacter freundii
Internal control
Ct values
PP1/MM1
PP2/MM1
PP3/MM1
PP1/MM2
PP2/MM2
PP3/MM2
PP1/MM3
PP2/MM3
PP3/MM3
0
5
10
15
20
25
30
35
Salmonella abony
Salmonella enteritidis Citrobacter freundii
Internal control
Ct
values
Prep Lot1
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Res arch Institute
Expert Review Panel Use Only