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Figure7: Summary data: Top: Green channel (
Salmonella
detection) Lower:
Yellow channel (internal control)
Figure7
:
Ct values from the accelerated aging studies showed no slope over the course of the
120 day accelerated aging study in either the
Salmonella
(green) detection channel or the
internal control (yellow) channel. There was a weak green signal in the
C. freundii
channel at
120 days, attributed to cross contamination in the culture. (TTP: Test time point)
Method developer summary data shelf life QIAsymphony DNA Midi Kit
The QIAsymphony Bacteria Kit used in these studies is based on the
QIAsymphony DNA Midi Kit. All cartridge chemicals are identical, except for the
Proteinase K that is included in the QIAsymphony DNA Midi Kit but omitted from
the QIAsymphony Bacteria Kit. Proteinase K is not required for the simple heated
lysis implemented in the QIAsymphony Bacteria Kit. It is used in the reference
DNA Midi Kit to disrupt the membranes of animal and human cells and tissues.
The inclusion of the Proteinase K in the kits for functional testing did not show
any changes over the storage of the DNA Midi Kit. One can reasonably assume
that the other components of the parent kit (magnetic beads, lysis, binding and
elution buffers) that remain constant between the kits would be stable for the
same 24 month period. We are providing stability data on the QIAsymphony DNA
20
25
30
35
40
0 d
10 d
20d
40d
60d
90d
120d
Ct
Time(days)
Green all TTP Stability test
Salmonella spp
.
Salmonella enteritidis
Salmonella abony
Citrobacter freundii
20
25
30
35
40
0 d
10 d
20d
40d
60d
90d
120d
Ct
Time (days)
Yellow allTTP Stability test
Salmonella spp
.
Salmonella enteritidis
Salmonella abony
Citrobacter freundii
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only