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Figure 5: Summary of sample prep lot variation Automated sample prep

Figure 5

:

Ct values for

Salmonella abony, Salmonella enteritidis, C. freundii

and the internal

control were not significantly different, indicating that there were no effects of the lot of the

QIAsymphony Bacteria kit on the detection.

Figure 6: Rotor-Gene Q instrument variation with automated sample prep

Figure 6

: Ct values for

Salmonella abony, Salmonella enteritidis, C. freundii

and the

internal control were not significantly different, indicating that there was no effect of the

Rotor-Gene Q instruments on the results obtained

0

5

10

15

20

25

30

35

40

Salmonella abony

Salmonella enteritidis

Citrobacter freundii

Internal control

Ct

Bacterial strain

Prep Lot1

Prep Lot2

Prep Lot3

0

5

10

15

20

25

30

35

40

Salmonella abony

Salmonella enteritidis

Citrobacter freundii

Internal control

Ct

Bacterial Strain

Cycler 1

Cycler 2

OMAMAN-36 E/ AOAC PTM Report

ERP Use Only

January 2017

AOAC Research Institute

Expert Review Panel Use Only