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Table 2014.04E. Mass spectrometer (Xevo TQ-S) operating conditions
Ionization mode
ESI positive
Capillary voltage
2.0 kV
Collision gas pressure
2–5 × 10
-3
mtorr
Source temperature
150°C
Source offset
30 V
Desolvation temperature 550°C
Cone gas flow
300 L/h
Desolvation flow rate
1000 L/h
Peak width at half height
0.7 amu
a
a
Instrumental resolution parameters for the TQ-S are set up by IntelliStart to
achieve a resolution of approximately 0.7 amu across the mass range.
Table 2014.04F. Chromatographic parameters
Mobile phase A
10 mM ammonium acetate [water-acetonitrile (95 + 5, v/v)]
Mobile phase B
10 mM ammonium acetate [water-acetonitrile (5 + 95, v/v)]
Flow rate
0.7 mL/min (analytical)
Flow rate into MS
Full flow
Column
Acquity UPLC BEH HILIC 1.7 µm, 2.1 × 100 mm
Column temperature 25°C
Injection volume
10 µL
Injection type
Full loop
Sample temperature 8°C
Candidates for 2016 Method of the Year
318