381 / 471
Table 2014.04G. Gradient profile
Time, min
Flow, mL/min
Mobile phase A, %
Mobile phase B, %
Curve
Initial
0.7
8.0
92.0
0.10
0.7
8.0
92.0
6
6.00
0.7
22
78
6
6.01
0.7
100
0
6
8.00
0.7
100
0
6
10.00
1.0
8.0
92.0
6
13.00
1.0
8.0
92.0
6
13.20
0.7
8.0
92.0
6
15.00
0.7
8.0
92.0
6
Candidates for 2016 Method of the Year
319