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resolution in all three dimensions – resulting in a 5x smaller confocal
volume. Or push the sensitivity beyond the limits of all conventional
confocal microscopes; or use the increase in signal-to-noise ratio to speed
up your image acquisition.
Traditionally, deeply imaging into intact tissue typically requires multi-
photon excitation to penetrate deeper than near the surface of a tissue.
Using a “clearing” method to remove the light obstructing opaque
molecules from a tissue has been another technique for deep imaging.
Techniques such as SCALE, CLARITY, ClearT, SeeDB, CUBIC and others
have allowed researchers to image deeper than a millimeter into cleared
animal model brains and organs.
ZEISS Lightsheet Z.1 features high speed image acquisition and greatly
reduced photo damage making imaging of live developmental samples
and fixed and cleared tissues easier than ever before. Come learn about
using the innovative ZEISS Lightsheet Z.1 microscope for imaging of fixed
and cleared tissues.
Speakers
Joseph Huff, Product Marking Manager, Laser Scanning and Superresolu-
tion Microscopy, Carl Zeiss Microscopy LLC
Scott Olenych, Product Marketing Manager, Imaging Products, Carl Zeiss
Microscopy LLC
International Relations Committee Meeting
10:30 am - 12:00 pm, Room 410
Symposium
New and Notable
10:45 am - 12:45 pm, Petree Hall C
Co-Chairs
Vasanthi Jayaraman, University of Texas Health Science Center, and
E. Michael Ostap, University of Pennsylvania
No Abstract
10:45 am
STRUCTURE OF A TRPV2 ION CHANNEL,
Seok-Yong Lee
No Abstract
11:02 am
CRYO-EM STRUCTURE OF A MICRONUTRIENT TRANSPORTER WITH UN-
USAUL ARCHITECTURE.
Filippo Mancia
No Abstract
11:19 am
STRUCTURAL DYNAMICS OF K CHANNEL GATING REVEALED BY SINGLE
MOLECULE FRET.
Colin Nichols
No Abstract
11:36 am
THE MOTIONS AND INTERACTIONS IN MISMATCH REPAIR TAGET SEARCH
DYNAMICS ARE REVEALED BY LIVE-CELL SINGLE-MOLECULE MICROS-
COPY.
Julie Biteen
No Abstract
11:53 am
INTEGRATIVE STRUCTURAL BIOLOGY OF TETRAHYMENA TELOMERASE.
Juli Feigon
No Abstract
12:10 am
INTRINSICALLY DISORDERED PROTEINS AS PHYSICAL DRIVERS OF MEM-
BRANE TRAFFIC.
Jeanne C. Stachowiak
No Abstract
12:27 pm
FLOPPY BUT NOT SLOPPY: DECODING PLASTICITY IN THE DARK PRO-
TEOME OF THE NUCLEAR TRANSPORT MACHINERY.
Edward Lemke
Symposium
Computational and Experimental Approaches
to Protein Design
10:45 am - 12:45 pm, Petree Hall D
Chair
Rama Ranganathan, University of Texas, Southwestern
118-Symp10:45 am
MAPPING CONSTRAINTS ON PROTEIN EVOLUTION.
Jesse Bloom
119-Symp11:15 am
UNCOVERING AND REPROGRAMMING G PROTEIN COUPLED RECEPTOR
SIGNALING.
Patrick Barth
120-Symp11:45 am
DESIGNING PEPTIDE INHIBITORS OF ANTI-APOPTOTIC BCL-2 FAMILY
PROTEINS.
Amy E. Keating
No Abstract
12:15 pm
PROTEIN MECHANICS: THE LINK BETWEEN STRUCTURE AND FUNCTION.
Rama Ranganathan
Platform
Cryo-Electron Microscopy
10:45 am - 12:45 pm, Room 502A
Co-Chairs
Gunnar Schroeder, Forschungszentrum Juelich, Germany
Sharon Wolf, Weizmann Institute of Science, Israel
121-Plat
10:45 am
HIGH-RESOLUTION STRUCTURAL INSIGHT INTO THE MYOSIN VI-F-ACTIN
INTERFACE. Laura Y. Kim, Pinar S. Gurel, Tosan Omabegho, Zev Bryant,
Gregory M. Alushin
122-Plat
11:00 am
USING CRYO-EM TO UNTANGLE THE CONFORMATIONAL LANDSCAPE
OF A SMALL ALLOSTERICALLY-REGULATED COMPLEX.
Mario J. Borgnia
,
Soojay Banerjee, Alberto Bartesaghi, Doreen Matthies, Prashant Rao,
Alan Merk, Jason Pierson, Jacqueline L. Milne, Sriram Subramaniam
123-Plat
11:15 am
THE STRUCTURE OF THE METHANOSPIRILLUM HUNGATEI FLAGELLUM AS
DETERMINED BY CRYO ELECTRON MICROSCOPY.
Nicole Poweleit
124-Plat
11:30 am
IN SILICO REDUCTION OF CONFORMATIONAL VARIANCE IN CRYO-EM
IMAGING.
Gunnar F. Schröder
, Michaela Spiegel, Amudha Kumari
Duraisamy
125-Plat
11:45 am
COLLAPSE OF INDIVIDUAL DNA CHAINS CONFINED IN BACTERIOPHAGE
CAPSIDS.
Françoise Livolant
, Amélie Leforestier, Marta De Frutos,
Dominique Durand
126-Plat
12:00 pm
STRUCTURAL REMODELING OF BACTERIOPHAGE Φ29 DURING INFEC-
TION OF GRAM-POSITIVE BACTERIUM.
Madeline M. Farley
, Jiagang Tu,
Ian Molineux, Jun Liu
127-Plat
12:15 pm
CRYO-STEM TOMOGRAPHY PROVIDES MORPHOLOGICAL AND CHEMICAL
CHARACTERIZATION OF PRECIPITATED CALCIUM-PHOSPHATE CLUSTERS
SEQUESTERED IN MITOCHONDRIA OF INTACT VITRIFIED FIBROBLASTS.
Sharon G. Wolf
, Yael Mutsafi, Ben Horowitz, Michael Elbaum, Deborah
Fass
128-Plat
12:30 pm
ELECTRON CRYO-TOMOGRAPHY OF NANOWIRES IN SHEWANELLA
ONEIDENSIS MR-1.
Poorna Subramanian
, Sahand Pirbadian, Mohamed
Y. El-Naggar, Grant J. Jensen