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originating from two proteins in each allergenic

food. However, the quantitative method

information seems to indicate that only one peptide

from each food is used for quantification (with two

corresponding transitions). How are calculations

conducted if different peptides disagree on

quantification or qualitative presence?

1. Does the applicability of

the method support the

applicability of the SMPR?

If not, please explain what

is missing.

Yes

2. Does the analytical

technique(s) used in the

method meet the SMPR?

If not, please specify how

it differs from what is

stated in the SMPR.

Yes

3. Are the definitions

specified in the SMPR

used and applied

appropriately in the

method? If no, please

indicate how the terms

are used.

No. In several cases the authors appear to use

different units than those indicated in the SMPR.

Specifically, the authors use whole milk powder

instead of whole fluid milk, spray dried whole egg

instead of whole liquid egg, and peanut butter

instead of peanuts. The differences in these units

can result in up to an 8-fold difference in

concentration, as is the case with whole milk

powder vs. whole fluid milk. In some instances,

these differences would result in failure to meet the

method performance requirements. For example,

the stated MQL of 3 ppm whole milk powder in

cookie would equate to approximately 24 ppm

whole fluid milk in cookie. In addition, the

presentation of separate results for egg white and

egg yolk are not in keeping with the SMPR, which

calls for the detection and quantification of whole

egg.