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- The peptides are, however, shown to be from the
correct proteins in Appendix 1.
Milk:
- The authors do indicate two peptides from Bos d 9
(alpha-s1-casein). However, the other two peptides
given for milk are from different proteins.
Milk.Protein_2.Peptide_A is from Bos d 5 (beta-
lactoglobulin) and Milk.Protein_2.Peptide_B is from
Bos d 11 (beta-casein). Despite the name given to
the peptides, they are not from the same protein.
For milk, the authors therefore have two peptides
for one protein and only one peptide for two
additional proteins. In the case of the beta-
lactoglobulin and beta-casein peptides, there is only
one quantifier peptide and one qualifier peptide
between these two proteins.
- Again, the peptides are, shown to be from the
correct proteins in Appendix 1.
In addition, while the authors state that a total of
eight MRM transitions for each allergen commodity
are used in the method, validation seems to have
only been conducted for one peptide (two
transitions) for each commodity-matrix
combination. What happens when the quantifier
ion is detected but the qualifier ion(s) is/are not?
It is unclear from the description of the preparation
of the incurred/spiked foods whether all of the
target allergenic foods (milk, egg, peanut, and
hazelnut) were incurred/spiked into the same batch
of the food matrix (e.g. cookie) or whether each
allergenic food was incurred into separate batches
of the food matrices.
The authors also do not consistently and clearly
state all units. Due to the differences in potential
differences, all units must be complete and specific,