AOAC SPSFAM ERP for SELECTED FOOD ALLERGENS

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Submission Date
Name
E-mail
Organization
Title of Method
AOAC Candidate Method
Number (e.g. ALN-01)
Applicable SMPR
2. Does the analytical
technique(s) used in the
method meet the SMPR? If
not, please specify how it
differs from what is stated in
the SMPR.
3. Are the definitions
specified in the SMPR used
and applied appropriately in
the method? If no, please
indicate how the terms are
used.
2017-02-28 12:44:08
Tomasz Tuzimski
Medical University in Lublin, Poland
Detection and Quantitation of Selected Food Allergens using LC-MS/MS (Revision 2)
ALL-01 (Revision 2)
2016.002
The proposed method entitled ‘Detection and Quantitation of Selected Food Allergens
using LC-MS/MS (Revision 2)’ described by Lee Sun New, Andre Schreiber and Hua-
Fen Liu is applicable for the detection and quantitation of egg, milk, peanut, and
hazelnut food allergens in finished food products and ingredients by LC-MS/MS.
The method uses triple quadrupole mass spectrometry and selective Multiple Reaction
Monitoring (MRM) of characteristic transitions of precursor ions to fragment ions of
multiple proteins and peptides to uniquely identify each allergen. Characteristic
signature peptides were chosen for each alergen, and MRM transitions for each
signature peptide were determined based on their uniqueness compared to
background proteins and their sensitivity of detection. For each allergen multiple
unique peptides were chosen from unique proteins, and two MRM transitions per
peptide were chosen.
The method performance requirements were met for the detection of egg, milk, peanut
and hazelnut in a number of food matrices.
The method can be easily extended to the detection of other allergens, including soy
and other tree nuts (almonds, Brazil nut, cashew, pine nut, pistachio, pecan, and
walnut). The method was verified in-house and across two other different laboratories.
YES: The applicability of the method is adequate to the applicability of the SMPR.
The target commodity analytical range of 10 ‒1000 ppm for all matrices with recoveries
(refer to Definitions) between 60‒120% and RSDr of less than 20% were achieved for
all allergen commodities in the food matrices required for AOAC SMPR 2016.002.
YES: The analytical techniques in the method are adequate and meet the SMPR.
YES: Definitions, which are specified in the SMPR, were listed in the description, also
were applied appropriately in the method.
AOAC SPSFAM ERP REVIEW FORM - MARCH 13, 2017
I. General Summary:
Does the applicability of
the method support the
applicability of the SMPR? If
not, please explain what is
missing.
II. Review of the Method Only:
1