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2. Is there information
demonstrating that the
method system suitability
tests and controls as
specified in the SMPR worked
appropriately and as
expected? If no, please
specify.
3. Based on the supporting
information, is the method
written clearly and concisely?
If no, please specify the
needed revisions.
4. Based on the supporting
information, what are the
pros/strengths of the
method?
5. Based on the supporting
information, what are the
cons/weaknesses of the
method?
6. Any general comments
about the method?
Do you recommend this
method be adopted as a First
Action and published in the
Official Methods of Analysis
of AOAC INTERNATIONAL?
Please specify rationale.
YES: There are information demonstrating that the method system suitability tests and
control as specified in the SMPR worked appropriately and expected.
The method is well described and substantively prepared. The project of the method is
well integrated and includes a clear and concise description.
The developed method was evaluated following the definitions of AOAC SMPR
2016.002 with respect to Method quantitation limit (MQL), Method detection limit
(MDL), Linearity, Repeatability, Reproducibility, Recovery.
Specificity is another important analytical parameter. Characteristic signature peptides
were chosen for each allergen, and MRM transitions for each signature peptide were
determined based on their uniqueness compared to background proteins and their
sensitivity of detection.
Page 49, Table (iv) Hazelnut commodity:
For 10 ppm (10000 ppb), information is given that analyte concentrations are BQL
(below method quantification limit).
Does the procedure fulfil the method criteria?
The cons/weakness of the method may be costs. But I think it is inevitable.
Page 42, Table 9:
Despite the fact that mean values of SD and RSD% are less than 16.7%, recovery
values, as well as SD and RSD%, ought to be provided for all three spiking levels of
10, 100 and 1000 ppm separately.
In my opinion, the Method #ALL-01 entitled: Detection and Quantitation of Selected
Food Allergens using LC-MS/MS (Revision 2) described by Lee Sun New, Andre
Schreiber and Hua-Fen Liu is applicable for the detection and quantitation of egg, milk,
peanut, and hazelnut food allergens in finished food products and ingredients by LC-
MS/MS. The revised (January 2017) Method #ALL-01 should be adopted in its present
form as a First Action and recommended for publication in the Official Methods of
Analysis of AOAC INTERNATIONAL.
V. Final Recommendation