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Biophysical Society 59
th
Annual Meeting, Baltimore, Maryland
z-stacking in various biological systems such as cells, tissue, drosophila, C.
elegans, bacteria and virus makes the Vutara 350 very versatile.
Presenter
Jeff Stuckey, Product Marketing Manager, Bruker Nano Surfaces
3:30
pm
–5:00
pm
, R
oom
333
Early Careers Committee Meeting
3:30
pm
–5:00
pm
, H
all
C, R
oom
A
Exhibitor Presentation
Wyatt Technology Corporation
The Light Scattering Toolkit for Biophysical Characterization: Lab
Essentials for Enhancing Studies of Purification, Crystallization,
Formulation, Conjugation, Conformation, and Interactions
Biophysical techniques based on static and dynamic light scattering
address many of the key analytical challenges associated with proteins,
oligonucleotides, vesicles and other biomacromolecules. This workshop
covers the following topics:
1. Batch DLS – traditional cuvette-based dynamic light scattering (DLS)
is a fast, easy means of estimating macromolecular and nanoparticle size
distributions to assess protein aggregation or the sizes of virus-like particles
or drug delivery nanovehicles. In microwell-plate format, DLS is a high-
productivity tool useful for optimizing formulation or crystallization
conditions with minimal sample consumption or manual labor.
2. SEC-MALS and SEC-DLS – coupling of multi-angle static light
scattering (MALS) and DLS detection to size-exclusion chromatography
to assess molar mass, size, conformation and conjugation, in
solution, independently of column calibration and non-ideal sample-
column interactions. In addition to readily assessing aggregation and
fragmentation in line with SEC purification, SEC-MALS analyzes
protein conjugates such as glycoproteins or membrane proteins bound to
surfactant micelles, determining protein oligomeric state and the mass of
glycans, polysaccharides or surfactant modifying the protein.
3. FFF-MALS and FFF-DLS – coupling of MALS and DLS to a field-
flow fractionation (FFF) device to achieve accurate characterization of
macromolecules and nanoparticles from 1-1000 nm, even when soluble
and insoluble components are both present in the solution. It does not
employ a stationary phase; FFF separates without shear and with minimal
surface interactions. FFF produces high-resolution size distributions
thanks to true hydrodynamic separation upstream of the light scattering
detectors. It also offers the benefits of post-separation downstream analysis
by spectroscopy for additional information on samples.
4. CG-MALS – coupling MALS to a composition-gradient device results
in a uniquely powerful system for characterizing complex biomolecular
interactions, label-free and immobilization-free. Because MALS measures
molar masses it is one of the most useful techniques for analyzing
multi-domain, multi-protein interactions that go beyond standard 1:1
interactions including systems exhibiting cooperativity and allostery. CG-
MALS determines the affinity and absolute molecular stoichiometry of self
and/or heteroassociating systems from pM to mM.
Presenter
Stephanie Cope, Applications Scientist, Wyatt Technology Corporation
4:00
pm
–5:00
pm
, R
oom
301/302/303
Career Center Workshop
Beyond the Bench: Preparing for Your Career
Transition in the Life Sciences
There are numerous alternative career options for the seasoned bench
scientist who may have decided to take his/her talents and apply them in
a new direction. This transition can be accomplished without having to
matriculate in another graduate program, and this session explores the
how’s and why’s of making such a transition. Be prepared to talk about the
role you are thinking about moving into, why you may have chosen this
alternative path, and what successes you may have had thus far.
4:00
pm
–6:00
pm
, B
allroom
I
Symposium
Emergent Properties and Collective
Behaviors of Complex Systems
Chair
Aaron Dinner, University of Chicago
158-S
ymp
4:00
pm
SCALING LAWS GOVERNING GROWTH AND DIVISION OF
SINGLE BACTERIAL CELLS.
Aaron Dinner
159-S
ymp
4:30
pm
DRIVING WITH THE BRAKES ON: AN INCOHERENT
TRANSCRIPTIONAL CIRCUIT PATTERNS THE DROSOPHILA
EMBRYO.
Angela DePace
160-S
ymp
5:00
pm
TEMPORAL FREQUENCY OF DIRECTIONAL SENSING AND
COLLECTIVE MIGRATION IN DICTYOSTELIUM.
Satoshi Sawai
161-S
ymp
5:30
pm
THE EMERGENCE OF HEART FAILURE AS A CONSEQUENCE
OF MYOCARDIAL METABOLIC DYSFUNCTION.
Daniel A. Beard
4:00
pm
–6:00
pm
, B
allroom
II
Symposium
Protein Evolution and Allosteric Networks
Chair
Corey Wilson, Yale University
162-S
ymp
4:00
pm
UNDERSTANDING ENZYME MOLECULAR EVOLUTION
TOWARD THERMAL ADAPTATION USING MULTISTATE
COMPUTATIONAL PROTEIN DESIGN.
Corey J. Wilson
163-S
ymp
4:30
pm
ALLOSTERIC NETWORKS IN THROMBIN.
Elizabeth Komives
164-S
ymp
5:00
pm
THE EVOLUTION OF ENZYME MECHANISMS AND
FUNCTIONAL DIVERSITY.
Janet Thornton
N
o
A
bstract
5:30
pm
EXPERIMENTAL RECONSTRUCTION OF THE MECHANISMS
OF ANCIENT PROTEIN EVOLUTION.
Joe Thornton
4:00
pm
–6:00
pm
, B
allroom
III
Symposium
Cardiomyopathies and Contractile Proteins
Chair
Leslie Leinwand, University of Colorado
165-Symp
4:00
pm
MYOSIN MYOPATHIES.
Leslie Leinwand
166-Symp
4:30
pm
POSTTRANSLATIONAL MODIFICATION OF TITIN DOMAINS
AS A MAIN REGULATOR OF MYOCARDIAL STIFFNESS.
Wolfgang A. Linke
167-Symp
5:00
pm
MYBPC3 GENE THERAPY FOR NEONATAL SARCOMERIC CAR-
DIOMYOPATHIES.
Lucie Carrier