SPDS Lutein and Turmeric ERPs

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Reviewer Name: Elizabeth Mudge
Email:
Organization: BCIT
Method Reviewed: TUR‐02
Method Title: Quantitation of Curcuminoids
Applicable SMPR 2016.003
Summary of Method: This method is to quantify curcuminoids (curcumin, demethoxycurcumin and bis‐
demethoxycurcumin) in turmeric roots and finished products using HPLC with
detection at 425 nm.
1. Does the Applicability of the
Method Support the Applicability
of the SMPR? If not, please explain
what is missing.
Yes ‐ this method is applicable to quantitation of the three major curcuminoids in
turmeric roots and finished products
2. Does the analytical technique(s)
used in the method meet the
SMPR? If not, please specify how it
differs from what is stated in the
SMPR.
Yes.
3. Are the definitions specified in
the SMPR used and applied
appropriatly in the method? If no,
please indicate how the terms are
used.
Yes.
4. Does the method, as written,
contain all appropriate precautions
and warnings related to the
method's regaents, components,
instrumentation, or method steps
that may be hazardous? If no,
please suggest wording or
option(s).
Yes ‐ most of this method does not require significant safety warnings
1. Are the definitions specified in
the SMPR used and applied
appropriately in the supporting
documentation (manuscripts,
method studies, etc…)? If not,
please explain the differences and
if the method is impacted by the
difference.
For the most part, yes ‐ with the exception of repeatability. The studies performed
here specify 6 replicates, but in this case these are actually triplicate samples with
duplicate injections, treated as six replicates. This data is therefore somewhat biased
to improve the precision of the method because replicate injections of the same
sample will improve repeatability compared with six actual replicate samples which
take into account the entire sample preparation.
2. Is there information
demonstrating that the method
meets the SMPR Method
Performance Requirements using
the Reference Materials stated in
the SMPR? If not, then specify
what is missing and how this
impacts demonstration of
performance of the method.
No reference materials were used in this study report.
General Information
I. Summary
II. Review of the Method Only
III. Review of Information in Support of the Method