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7 . Any general comments about
the method?
The selection of extraction solvent seemed to be based more on the precision of the
replicates rather than on analyte recovery. When looking at the error, the error of
the methanol is slightly larger, but there is a significant difference between the
quantity of curcumin and DMC extracted with methanol compared with the 80%
methanol. Without any recovery data in this study, it appears that the accuracy of
this method may be insufficient. There were also re‐extraction repetitive dilution
study, but without descriptions of the actual methodologies, it is difficult to
understand how this graph explains that only 1 extraction is required.
Do you recommend this method be
adopted as a First Action and
published in the Official Methods
of Analysis of AOAC
INTERNATIONAL? Please specify
rationale.
No. The method appears to be missing key information including method
recovery/accuracy. The repeatability data was calculated using duplicate injections
as replicates and the selection of extraction solvent appears to impact the recovery
of the curcuminoids.
V. Recommendation for the Method