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AOAC Official Method 2015.10
Free and Total Choline and Carnitine
in Infant Formula and Adult/Pediatric Nutritional Formula
Liquid Chromatography/TandemMass Spectrometry (LC/MS/MS)
First Action 2015
A.
Apparatus
(a)
LC system
. – Prominence (Shimadzu, Kyoto, Japan) or equivalent.
(b)
MS/MS system
. – API 4000 with Electrospray Ionization (ESI) (ABSciex, Framingham, MA) or
equivalent.
(c)
Mass Spectrometry Software
. – Analyst (ABSciex) or equivalent.
(d)
Analytical column
. – Zorbax 300-SCX, 3.0 x 50mm, 5 µm (Agilent, Santa Clara, CA) or equivalent.
(e)
Microwave
. –MARS6 (CEM, Mathews, NC) or equivalent.
(f)
Microwave turntable, liner, and cap
. –MARSXpress, 55mL PFA Teflon®, 40 position (CEMor
equivalent).
(g)
Vortex mixer.
– (VWR, West Chester, PA) or equivalent.
(h)
Analytical balances.
–Model CPA225D (Sartorius, Goettingen, Germany) or equivalent.
(i)
Horizontal shaker.
–Model 6010 (Eberbach, Ann Arbor, MI) or equivalent.
(j)
Magnetic stir plate.
–Model PC-420D (Corning, Corning, NY) or equivalent.
(k)
Positive displacement pipets.
–Microman, various sizes (Gilson, Middleton, WI) or equivalent.
(l)
Repeater positive displacement pipet
. – Repeater Plus (Eppendorf, Hamburg, Germany) or
equivalent.
(m)
Polypropylene tubes
. – Digitube, assorted sizes (SCP Science, Montreal, Canada) or equivalent.
(n)
Mobile phase containers
. – 2 L glass (VWR) or equivalent.
(o)
Syringe filters
. – 0.45 µm PTFE and GHP (Pall, Plano, TX) or equivalent.
(p)
Disposable syringes
. – 3mL (BD Biosciences, Franklin Lakes, NJ) or equivalent.
(q)
Graduated cylinders
. – Assorted sizes (VWR) or equivalent.
(r)
Magnetic stir bars
. – 7.9 x 50mm (VWR) or equivalent.
(s)
Autosampler vials/caps
. – 1.5mL silanized crimp top (VWR) or equivalent.
(t)
Microcentrifuge tubes
. – 1.5mL polypropylene (VWR) or equivalent.
(u)
Bottle top dispenser
. – 5mL acid resistant (Brand, Essex, CT) or equivalent.
(v)
Desiccator
. – glass (VWR) or equivalent.
Candidates for 2016 Method of the Year
117