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29
of
76
indicating no significant difference between the candidate presumptive
and confirmed results.
For the high inoculation level, a dPOD
C
value of 0.00 was obtained with a
95% confidence interval of (-0.43, 0.43), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.43,
0.43), indicating no significant difference between the candidate
presumptive and confirmed results. Detailed results of the POD analyses
are presented in Tables 15 and 19 of the Appendix.
QIAsymphonyDNA Extraction Procedure- E. coli Screen Plus
Fresh Spinach
For the low inoculation level of the
mericon
E. coli O157 Screen
PlusPathogen Detection Assay, there were 7 presumptive positives and 7
confirmed positives following the FDA/BAM Chapter 4A reference
method confirmation procedure. There were 9 observed positives for the
reference method. For the high inoculation level, there were 5
presumptive positives and 5 confirmed positives following the FDA/BAM
Chapter 4A reference method confirmation procedure. There were 5
confirmed positives following the reference method.
For the low inoculation level, a dPOD
C
value of -0.10 was obtained with a
95% confidence interval of (-0.37, 0.19), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.28,
0.28), indicating no significant difference between the candidate
presumptive and confirmed results.
For the high inoculation level, a dPOD
C
value of 0.00 was obtained with a
95% confidence interval of (-0.43, 0.43), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.43,
0.43), indicating no significant difference between the candidate
presumptive and confirmed results. Detailed results of the POD analyses
are presented in Tables 14 and 18 of the Appendix.
Raw Ground Beef (70% Lean)
For the low inoculation level of the
mericon
E. coli O157 Screen
PlusPathogen Detection Assay, there were 9 presumptive positives and 9
confirmed positives following the USDA/FSIS-MLG 5.09 reference
method confirmation procedure. There were 9 observed positives for the
reference method. For the high inoculation level, there were 5
presumptive positives and 5 confirmed positives following the
USDA/FSIS-MLG 5.09 reference method confirmation procedure. There
were 5 confirmed positives following the reference method.
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only