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Figure 1: Primer-probe and master mix lot variation for
mericon
E. coliO157
Screen Plus assay.
Ct values for all primer/probe (PP) and Master Mix (MM) lot combinations for
the
mericon
E. coliO157Screen Plus Pathogen Detection Assaywere not
significantly different, indicating that there were no effects of individual lots of
primer/probes and Master Mix on the detection results. The assay is a
multiplex giving results in the green channel for
stx1/stx2,
in the orange
channel for
O157¸
and in the crimson channel for
eae.
Results for
Citrobacter freundii
(a closely related but non-
E. coli
bacterial strain)are
shown.
Lot to Lot Study:
mericonE. coli STEC O-Type Pathogen Detection Assay
0
5
10
15
20
25
30
35
40
E.coli
O157:H7
E.coli
O157
E.coli
O26:H-
E.coli
O111:H- Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O157 Screen Plus
:
O157
PP1/MM1
PP2/MM1
PP3/MM1
PP1/MM2
PP2/MM2
PP3/MM2
PP1/MM3
PP2/MM3
PP3/MM3
0
5
10
15
20
25
30
35
40
E.coli
O157:H7
E.coli O157 E.coli
O26:H-
E.coli
O111:H-
Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O157 Screen Plus: eae
PP1/MM1
PP2/MM1
PP3/MM1
PP1/MM2
PP2/MM2
PP3/MM2
PP1/MM3
PP2/MM3
PP3/MM3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only