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Figure 5.
mericonE. coli
O157 Screen Plus Prep lot variation
Ct values for DNA prep combinations using consistent primer probe:master
mix lots for the
mericonE. coli
O157 Screen PlusPathogen Detection
Assaywere not significantly different, indicating that there were no effects of
different bacterial prep lots on the detection results
mericonE. coli
STEC O-type assay
Each Pathogen detection assay was evaluated for detection consistency with
each of the probes in the multiplex mixture (
Big 6 STEC and O157:H7)
using
3 different lots of the
mericon
DNA Bacteria Plus kits.
0.00
5.00
10.00
15.00
20.00
25.00
30.00
Ct
Bacterial strain
mericon
E. coli O157
Screen Plus: O157
Sample prep
prep 1
prep 2
prep 3
0.00 5.00 10.00 15.00 20.00 25.00 30.00
E.coli
O157:H7
E.coli O157 E.coli
O111:H-
E.coli
O126:H2
E.coli O145 Citrobacter
freundii
Ct
Bacterial strain
mericon
E. coli O157
Screen Plus: eae
Sample prep
prep 1
prep 2
prep 3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only