AOAC-RI ERP Book MICRO.pdf

Page

automated sample preparation and automated PCR preparation to minimize contamination.

The

mericon

kitsallow the end user a choice of two DNA extraction methods. The

automated

QIAsymphony

preparation method allows a high volume of samples to be

processed with minimal supervision utilizing easy to use software with an option to follow

step-by-step guidance through the sample prep workflow.

Conclusion

The data from these studies, within their statistical uncertainty, support the product claims

of the

mericon

E. coli O157Screen Plus and

mericon

E. coliSTEC O-TypePathogen

Detection Assaysfor the selected food matrixes tested, namely fresh spinach, raw ground

beef, and raw beef trim.

Results obtained from POD analysis in the method comparison study demonstrated no

statistically significant differences between the number of positive samples detected by the

candidate method, using the manual or automated DNA extraction procedures, and the

reference methods for fresh spinach, raw ground beef, and raw beef trim.

OMAMAN-36 E/ AOAC PTM Report

ERP Use Only

January 2017

AOAC Research Institute

Expert Review Panel Use Only