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Figure 6
.mericonE. coli
STEC O-type assay: Prep lot variation
Figure 6.
mericon
E. coli STEC O-type Prep lot variation
Ct values for DNA prep lots using consistent primer probe:master mix lots for
the
mericonE. coli
STEC O-type Pathogen Detection Assaywere not
significantly different, indicating that there were no effects of different
bacterial prep lots on the detection results
Stability Study: Lot stability
In this study, a single lot of each of the primer probe assay kits (
E. coli
O157Screen Plusand
E. coil
STEC O-type)was stored at 4 + 1
o
C for 4 and
15 months. A second set of kits from the same lot was stored at 37 + 1
o
C for
3 and 4.5 months. The 2 sets of primer probes stored at 37 + 1
o
C extends the
0.00 5.00 10.00 15.00 20.00 25.00 30.00
E.coli
O157:H7
E.coli O157 E.coli
O26:H-
E.coli
O111:H-
E.coli O145 Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O-type O157:H7: Lot-to-Lot prep
Prep 1
Prep 2
Prep 3
0 5 10 15 20 25 30
E.coli
O157:H7
E.coli O157 E.coli O26:H-
E.coli
O111:H-
E.coli O145 Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O-type Big 6: Lot-to-Lot prep
Prep 1
Prep 2
Prep 3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only