400 / 453
Page
38
of
76
Figure 3: Cycler variation for the mericon E. coli O157 Screen Plus assay
Ct values for all Cycler combinations using consistent primer probe:master
mix lots for the
mericon
E. coliO157 Screen Plus Pathogen Detection
Assaywere not significantly different, indicating that there were no effects of
different cyclers on the detection results.
mericon
E. coli STEC O-type assay
Each Pathogen detection assay was evaluated for detection consistency with
each of the probes in the multiplex mixture (
Big 6 STEC and O157:H7)
using
3 different Rotor-Gene Q cyclers.
Figure 4.
mericon
E. coli STEC O-type assayusing 3 Rotor-Gene Q cyclers
0.00
5.00
10.00
15.00
20.00
25.00
30.00
E.coli
O157:H7
E.coli O157 E.coli
O26:H-
E.coli
O111:H-
Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O157 Screen Plus
: eae
Cycler
RG1
RG2
RG3
0
5
10
15
20
25
30
E.coli
O157:H7
E.coli O157 E.coli
O26:H-
E.coli
O111:H-
E.coli O145 Citrobacter
freundii
Ct
Bacterial Strain
mericon®
E. coli STEC O-type
:
O-type
Cycler
RG1
RG2
RG3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Us Only