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Primer probe: Master Mix lot variation
Each Pathogen detection assay was evaluated for detection consistency with
each of the probes in the multiplex mixture (
stx1/stx2; eae,
and O157).
Figure 2.
mericon
E. coliSTEC O-type
Figure 2: Primer-probe and master mix lot variation for
mericon
E. coliSTEC
O-typeassay
Ct values for all primer/probe (PP) and Master Mix (MM) lot combinations for
the
mericon
E. coli STEC O-type Pathogen Detection Assaywere not
significantly different, indicating that there were no effects of individual lots of
primer/probes and Master Mix on the detection results. The assay is a
multiplex giving results in the green channel for the non-O157 Big 6 STEC O-
0 5 10 15 20 25 30
E.coli
O157:H7
E.coli
O157
E.coli
O26:H-
E.coli
O111:H-
E.coli
O145 Citrobacter
freundii
Ct
Bacterial Strain
mericon®
E. coli STEC O-type
: O-type
PP1/MM1
PP2/MM1
PP3/MM1
PP1/MM2
PP2/MM2
PP3/MM2
PP1/MM3
PP2/MM3
0.00
5.00
10.00
15.00
20.00
25.00
30.00
E.coli
O157:H7
E.coli O157 E. coli
O111:H-
E.coli O145 Citrobacter
freundii
Ct
Bacterial Strain
mericon®
E. coli STEC O-type: O157:H7
PP1/MM1
PP2/MM1
PP3/MM1
PP1/MM2
PP2/MM2
PP3/MM2
PP1/MM3
PP2/MM3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only