399 / 453
Page
37
of
76
types and in the orange channel for
O157:H7¸.
Results for
Citrobacter
freundii
(a closely related but non-
E. coli
bacterial strain)
,
are shown.
Consistency study: Effect of Cycler
mericon
E. coli O157 Screen Plus
Each Pathogen detection assay was evaluated for detection consistency with
each of the probes in the multiplex mixture (
stx1/stx2; eae, and O157)
using
3 different Rotor-Gene Q cyclers.
Figure3.
E. coli
O157 Screen Plus assay using 3 Rotor-Gene Q cyclers
0.00 5.00 10.00 15.00 20.00 25.00 30.00
E.coli
O157:H7
E.coli O157 E.coli
O26:H-
E.coli
O111:H-
Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O157 Screen Plus
: stx
Cycler
RG1
RG2
RG3
0.00
5.00
10.00
15.00
20.00
25.00
30.00
E.coli
O157:H7
E.coli O157 E.coli
O26:H-
E.coli
O111:H-
Citrobacter
freundii
Ct
Bacterial Strain
mericon
E. coli O157 Screen Plus
: O157
Cycler
RG1
RG2
RG3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only