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real time aging to approximately 18 and 24 months [9]. The same lot of
Master Mix was used for all time points from time 0 to 15
months.Todate,
only the t=o and 4 month timepoints have been completed.Reference DNA
was diluted from 10,000 copies/µl to 10 copies/µl
Figure 7. Stability
mericon
E. coli O157 Screen Plus assay
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00
t=0
4 months 4 months @37
Ct
Storage, months
Stability mericon
E. coli
Screen Plus:
stx1/stx2
10000 1000 100 25 10
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00
t=0
4 months 4 months @37
Ct
Storage, months
Stability mericon
E. coli
Screen Plus:
O157
10000 1000 100 25 10
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only