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Figure4. Cycler variation for
mericon
E. coliSTEC O-type assay.
Ct values for all Cycler combinations using consistent primer probe:master
mix lots for the
mericon
E. coliSTEC O-type Pathogen Detection Assaywere
not significantly different, indicating that there were no effects of different
cyclers on the detection results.
Consistency Study: Bacteria Plus Prep lot variation
Each Pathogen detection assay was evaluated for detection consistency with
each of the probes in the multiplex mixture (
stx1/stx2; eae, and O157)
using 3
different lots of the
mericon
DNA Bacteria Plus kits.
Figure 5
; mericon
E. coliO157 Screen Plus Prep lot variation
0.00
5.00
10.00
15.00
20.00
25.00
30.00
E.coli
O157:H7
E.coli O157 E.coli
O126:H2
E.coli O145 Citrobacter
freundii
Ct
Bacterial Strain
mericon®
E. coli STEC O-type
: O157:H7
Cycler
RG1
RG2
RG3
0.00 5.00 10.00 15.00 20.00 25.00 30.00
E.coli
O157:H7
E.coli
O157
E.coli
O111:H-
E.coli
O126:H2
E.coli
O145 Citrobacter
freundii
Ct
Bacterial strain
mericon
E. coli O157
Screen Plus: stx1/stx2
Sample prep
prep 1
prep 2
prep 3
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only