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For the low inoculation level, a dPOD
C
value of 0.00 was obtained with a
95% confidence interval of (-0.28, 0.28), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.28,
0.28), indicating no significant difference between the candidate
presumptive and confirmed results.
For the high inoculation level, a dPOD
C
value of 0.00 was obtained with a
95% confidence interval of (-0.43, 0.43), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.43,
0.43), indicating no significant difference between the candidate
presumptive and confirmed results. Detailed results of the POD analyses
are presented in Tables 14 and 18 of the Appendix.
Raw Beef Trim
For the low inoculation level of the
mericon
E. coli O157 Screen
PlusPathogen Detection Assay, there were 13 presumptive positives and
13 confirmed positives following the USDA/FSIS-MLG 5B.05 reference
method confirmation procedure. There were 13 observed positives for the
reference method. For the high inoculation level, there were 5
presumptive positives and 5 confirmed positives following the
USDA/FSIS-MLG 5B.05 reference method confirmation procedure. There
were 5 confirmed positives following the reference method.
For the low inoculation level, a dPOD
C
value of 0.00 was obtained with a
95% confidence interval of (-0.28, 0.28), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.28,
0.28), indicating no significant difference between the candidate
presumptive and confirmed results.
For the high inoculation level, a dPOD
C
value of 0.00 was obtained with a
95% confidence interval of (-0.43, 0.43), indicating no significant
difference between the candidate and reference methods. A dPOD
CP
value of 0.00 was obtained with a 95% confidence interval of (-0.43,
0.43), indicating no significant difference between the candidate
presumptive and confirmed results. Detailed results of the POD analyses
are presented in Tables 14 and 18 of the Appendix.
QIAsymphonyDNA Extraction Procedure- E. coli STEC O-Type
Fresh Spinach
For the low inoculation level of the
mericon
E. coliSTEC O-TypePathogen
Detection Assay, there were 7 presumptive positives and 7 confirmed
positives following the FDA/BAM Chapter 4A reference method
confirmation procedure. There were 9 observed positives for the
reference method. For the high inoculation level, there were 5
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institut
Expert Review Panel Use Only