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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
16
Table 4: Results of total Niacin as nicotinic acid estimated in
SPIFAN samples based on the analysis of total nicotinic acid
and nicotinamide, part II
Total Niacin as Nicotinic acid
mcg/100g of the reconstituted beverage or RTF
# 12 - Infant Elemental Powder
Average:
1489.6
%RSD:
5.8
n:
6
# 13 - Infant Formula Powder Milk based
Average:
968.8
%RSD:
5.0
n:
6
# 14 - Infant Formula Powder Soy based
Average:
1023.8
%RSD:
5.0
n:
6
# 15 -Infant Formula RTF Milk based
Average:
871.8
%RSD:
5.6
n:
6
# 16 - Adult Nutritional RTF High Protein
Average:
3065.1
%RSD:
5.5
n:
6
# 17 - Adult Nutritional RTF High Fat
Average:
4019.7
%RSD:
5.1
n:
6
The Total niacin values calculated based on the results of total
nicotinic acid and nicotinamide in the samples. The samples were
analyzed in triplicates independently on two days.
Accuracy of the method in estimation of total niacin (vitamin B3) assessed by analysis of NIST
SRM 1849a a milk based infant/adult nutrition formula sample. The results are presented in the
above Table 3. The method provided results which are within the NIST specifications for total
niacin for the NIST SRM sample. The precision of the analysis of the replicates of the sample
analyzed on multiple days (2 days) in terms of % RSD was found to be 5.0%.
VALIDATION REPORT
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