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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
13
Accuracy and Precision Evaluation of the method
Accuracy of the method in estimation of total niacin (vitamin B3) was evaluated by analysis of
NIST SRM 1849a a milk based infant/adult nutrition formula and NIST SRM 1546A a meat
sample. The results are presented in the following Table 1. The method provided results which
were within the NIST specifications for vitamin B3 for both of analyzed NIST samples. The
precision of the analysis of the replicates of the samples analyzed on multiple days (3 days) in
terms of % RSD was found to be 4.8% and 4.4% for infant/adult nutrition formula sample and
the meat sample respectively.
Table 1: Results of the analysis of the reference materials NIST 1849A
Infant/Adult
Nutritional Formula
& NIST 1546A Meat in replicates on multiple days (3 days) for
vitamin B3 contents
Total Vitamin B3
mcg/g
NIST 1849A
Adult/Infant
Nutritional Formula
NIST Value
108.13 +/- 9.92
Average:
105.4
% of NIST
Value
97.4
%RSD:
4.8
n:
7
NIST 1546A Meat
Homogenate
NIST Value
41 +/- 4.8
Average:
43.336
% of NIST
Value
105.7
%RSD:
4.4
n:
7
Analysis of SPIFAN Samples
Samples Details:
Samples analyzed in this study are listed in the following Table 2.
Table 2: Details of the SPIFAN Samples analyzed in the study
#
Samples for precision and
accuracy evaluation
Silliker Lab
ID
6
SRM 1849a
357621588
7
Adult Nutritional Powder Milk
Protein based
357621615
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE