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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
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3.) Chromatogram of an aliquot of a standard extracted and analyzed like a sample.
Fig 3: Chromatogram of a mixture vitamin B Standards processed through the method
like a sample. B6-PM (pyridoxamine), B1 (thiamine), B3-H (nicotinic acid), B3-N
(nicotinamide), B6-PL (pyridoxal), B6-PN (pyridoxine), B5 (pantothenic acid), B7
(biotin) and B12 (cyanocobalamin).
Chromatogram of the NIST 1849 Infant/adult nutrition formula provide no matrix
interference in analysis of nicotinic acid and nicotinamide in the sample (fig 4).
XICof +MRM(55pairs): 265.400/122.200DaID: B1_Quant fromSample13(NIST1849A) of 2016-03-03.wiff (Turb...
Max. 7951.0cps.
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0
1 45 88 132 175 219 263 306 350 393 437 481 524 568 611 655 699 742 786 830 873
Time, min
0.0
1.0e5
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
7.0e5
8.0e5
9.0e5
1.0e6
1.1e6
1.2e6
1.3e6
1.4e6
In te n s ity , c p s
B3-H
B3-N
B1
B6-PL
B7
B12
B5
B6-PN
B3-N
B6-PM
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE