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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
___________________________________________________________
Copyright © 2012 Silliker, Inc.
6
(
d
)
Flow rate. —
0.30 - 0.60 mL/min.
(
e
)
Injection volume. —
2.0
L.
(
f
)
Temperature. —
20
o
C.
(
g
)
Autosampler temperature. —
5.0
o
C.
(
h
)
Run time. —
21 min.
ABSciex 5500 QTrap / ABSciex 6500 QTrap
CUR – 30
CAD – medium
IonSpray Voltage IS – 4250
Temperature – 550
GS1 – 70
GS2 – 50
ID
Q1 Mass
(Da)
Q3 Mass
(Da)
Time
(msec)
DP
(volts)
EP (volts) CE (volts)
CXP (
volts)
B3-acid_Quant
124.3
78.1
20
50
10
28
9
B3-acid_Qual1
124.3
80
20
50
10
28
10
B3-acid_Qual2
124.3
53
20
50
10
29
10
B3-amide_Quant
123.3
80.1
20
45
10
27
10
B3-amide_Qual1
123.3
78.1
20
45
10
34
10
B3-amide_Qual2
123.3
53
20
45
10
35
10
B3-acid IS1
128.1
83
20
50
10
28
10
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE