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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
9
Fig 2: Chromatogram of a blank (contains IS) extracted like a sample through the process
and analyzed by LC-MS/MS.
An aliquot of a standard extracted and analyzed like a sample provided no interference in
the estmation of nicotinic acid and nicotinamide by the method (Fig 3).
XICof +MRM(55pairs): 265.400/122.200DaID: B1_Quant fromSample16(SampleBlank) of 2016-03-01.wiff (...
Max. 4.6e4cps.
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0
1 45 88 132 175 219 263 306 350 393 437 481 524 568 611 655 699 742 786 830 873
Time, min
0.0
1.0e5
2.0e5
3.0e5
4.0e5
5.0e5
6.0e5
7.0e5
8.0e5
9.0e5
1.0e6
1.1e6
1.2e6
1.3e6
1.4e6
1.5e6
1.6e6
1.7e6
1.8e6
1.9e6
2.0e6
In te n s ity , c p s
1.73
5.78
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE