AOAC SPIFAN Review Team (August 2016)

Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult

nutritional samples

___________________________________________________________

Chromatogram of the NIST 1849 Infant/adult nutrition formula

Fig 4: Chromatogram of NIST SRM 1849a (infant and adult nutrition formula) processed

through by the method for analysis of nicotinic acid and nicotinamide analysis and

estimation of total niacin. B6-PM (pyridoxamine), B1 (thiamine), B3-H (nicotinic acid),

B3-N (nicotinamide), B6-PL (pyridoxal), B6-PN (pyridoxine), B5 (pantothenic acid), B7

(biotin) and B12 (cyanocobalamin).

(b)

Calibration Curves obtained by the method

–

The calibration standards provided a good linear relationship between concentration of the

standards and their detector response for nicotinic acid as well as nicotinamide with R

values > 0.999.

XICof +MRM(55pairs): 265.400/122.200DaID: B1_Quant fromSample13(NIST1849A) of 2016-03-03.wiff (Turb...

Max. 7951.0cps.

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0

1 45 88 132 175 219 263 306 350 393 437 481 524 568 611 655 699 742 786 830 873

Time, min

0.0

1.0e5

2.0e5

3.0e5

4.0e5

5.0e5

6.0e5

7.0e5

8.0e5

9.0e5

1.0e6

1.1e6

1.2e6

1.3e6

1.4e6

In te n sity, cp s

B3-H

B3-N

B6-PL

B12

B6-PN

B3-N

B6-PM

VALIDATION REPORT

FOR ERP USE ONLY

DO NOT DISTRIBUTE