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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
14
8
Infant Formula Powder Partially
Hydrolyzed Milk based
357621671
9
Infant Formula Powder Partially
Hydrolyzed Soy based
357621721
10
Adult Nutritional Powder Low
Fat
357621752
11
Child Formula Powder
357621769
12
Infant Elemental Powder
357621776
13
Infant Formula Powder Milk
based
357621792
14
Infant Formula Powder Soy
based
357621812
15
Infant Formula RTF Milk based
(SPIFAN Control Milk)
357621827
16
Adult Nutritional RTF High
Protein
357621840
17
Adult Nutritional RTF High Fat
357621876
#
Samples for spike recovery
evaluation
1
Child Formula Powder
357621487
Reconstitution of powder samples for the analysis:
25 g of the powdered samples were taken into 200g water and mixed. The reconstituted
beverage was mixed thoroughly just before the analysis.
Analysis of the RTF sample:
The RTF samples were mixed and analyzed as is.
Results of the Niacin analysis in the SPIFAN Samples.
Total Nicotinic acid and Nicotinamide were analyzed in all the listed SPIFAN samples.
The powder samples were tested after their reconstitution as beverage as detailed earlier.
The RTF samples were tested as is. The total niacin in the samples was calculated based
on total nicotinic acid and nicotinamide in the samples. The samples were analyzed in
triplicates on two days (total n=6) in all the SPIFAN samples. NIST SRM was analyzed
in duplicated on two days (total n=40. It may be mentioned that the additional results for
the NIST SRM 1849a are provided in additional report provided along with this report.
The results are expressed as mcg of total niacin as nicotinic acid per 100 g of the
reconstituted or as is RTF samples. The results in case of the NIST SRM 1849a (one of
the SPIFAN samples) were calculated as mcg of total niacin as nicotinic acid per g of the
powdered sample so that results can be compared with the NIST specs for the sample.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE