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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
15
The results of the SPIFAN samples for total niacin (as nicotinic acid) are presented in the
following Tables 3 and 4.
Table 3: Results of total Niacin as nicotinic acid estimated in SPIFAN samples based on
the analysis of total nicotinic acid and nicotinamide, part I,
Total Niacin as Nicotinic acid
mcg/g of the powder
#6 - NIST SRM 1849A Adult/Infant Nutritional Formula
108.13 +/- 9.92
Average:
107.2
% Recov:
99.1 (Results % of NIST Expected value)
%RSD:
5.0
n:
4
Total Niacin as Nicotinic acid
mcg/100g of the reconstituted beverage or RTF
#7 - Adult Nutritional Powder Milk Protein based
Average:
710.2
%RSD:
3.5
n:
6
# 8 - Infant Formula Powder Partially Hydrolyzed Milk
based
Average:
754.4
%RSD:
5.4
n:
6
# 9 - Infant Formula Powder Partially Hydrolyzed Soy based
Average:
913.8
%RSD:
5.4
n:
6
# 10 - Adult Nutritional Powder Low Fat
Average:
1577.7
%RSD:
6.0
n:
6
# 11 - Child Formula Powder
Average:
581.5
%RSD:
6.0
n:
6
The Total niacin values calculated based on the results of total
nicotinic acid and nictoinamide in the samples. The samples were
analyzed in triplicates (NIST in duplicates0 independently on two
days.
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE