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Validation of a LC-MS/MS method of Analysis of Nicotinic acid and Nicotinamide in infant formula and adult
nutritional samples
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Copyright © 2012 Silliker, Inc.
8
Fig 1: Chromatogram of a mixture vitamin B Standards – approx 20 ppb. B6-PM
(pyridoxamine), B1 (thiamine), B3-H (nicotinic acid), B3-N (nicotinamide), B6-PL
(pyridoxal), B6-PN (pyridoxine), B5 (pantothenic acid), B7 (biotin) and B12
(cyanocobalamin).
A blank solution containing only internal standards extracted like a sample through the
process and analyzed by LC-MS/MS did not provide any interference in analysis of
nicotinic acid and nicotinamide by the method. Peak shape of the internal standards was
good.
2.) Chromatogram of a Sample – Blank (contains IS)
XICof +MRM(55pairs): 265.400/122.200DaID: B1_Quant fromSample9(std2) of 2016-03-03.wiff (TurboSpr...
Max. 1.3e6cps.
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 15.0 16.0 17.0 18.0 19.0 20.0
1 45 88 132 175 219 263 306 350 393 437 481 524 568 611 655 699 742 786 830 873
Time, min
0.0
2.0e5
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
1.6e6
1.8e6
2.0e6
2.2e6
2.4e6
2.6e6
2.8e6
3.0e6
In te n sity, cp s
1.73
B3-H
B3-N
B1
B6-PN
B6-PM
B6-PL
B5
B7
B12
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE